There is often confusion between dilution ratio (1:n meaning 1 part solute to n parts solvent) and dilution factor (1:n+1) where the second number (n+1) represents the total volume of solute + solvent. In scientific and serial dilution assays, the given dilution factor often means the ratio to the final volume, not to just the solvent. Dec 02, 2012 · No. Since all of your BSA samples were also same in volume, you need not multiply by a dilution factor. You use a dilution factor only when you add diluted sample (for. e.g. 1uL sample + 4uL H20, 2.5uL sample + 2.5uL H20 , etc etc.. ) Hope this helps.
If you are want to calculate dilution factor by hand. The 'titer' is the measurement of the amount of a substance in a solution. An antibody titer test is a blood test that doctors may use to help with diagnosis. Dilution factor (DF)= V(initial) / V (Final) Dilution after a seial dilution is given by multipying the dilutions for each step. 1mL sampl view the full answer Previous question Next question Transcribed Image Text from this Question
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